In this study for the first time, we demonstrated that the endogenous ligand of AHR, FICZ, has a positive effect on short- and long-term memory as well as learning skills. We also indicated that hippocampal dependent memory and learning task were improved by FICZ treatment and impaired by the AHR and Wnt/ß-catenin inhibition.
Our results showed that FICZ treatment led to elevation of the proneural transcription factors ASCL1 and Ngn2, immature neural marker DCX, differentiation neurons marker, NeuN, as well as β-catenin at mRNA and protein levels. To assess the hippocampal dependent memory, they also underwent contextual fear conditioning on the 28th day after treatment. qRT-PCR for gene analyses and protein assay on the 7th and 28th days were performed. Male BALB/C mice received FICZ, CH223191, and XAV-939 in a single dose of 100 μg/kg, 1 mg/kg, and 5 mg/kg of body weight respectively via intraperitoneal (IP) injection. In accordance, learning and hippocampus-dependent memory were examined. The neurogenesis effects of endogenously activated aryl hydrocarbon receptor (AHR) by its endogenous ligand, 6-formylindolo carbazole (FICZ), and its interactions with the Wnt/β-catenin signaling pathway were the main purpose of this study. It is regulated by multi-cellular micro-environment and different types of transcription factors. Neurogenesis is a dynamic and physiologic developmental process that affects learning and hippocampal dependent memory.
0 kommentar(er)
